The study on different acting mechanisms of three types of TACE inhibitors in converting of pro-TNF? into sTNF?
- VernacularTitle:三类TACE抑制剂的作用机理探讨
- Author:
Kongli ZHU
;
Yuzhen YANG
;
Ling HAN
- Publication Type:Journal Article
- Keywords:
TACE;
TNF?;
RDQ;
Anti-ODN;
Small molecular mimic peptide inhibitor
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of TNF? converting enzyme(TACE)inhibitors on TNF? secretion and develop an approach to interfere inflammation processes.Methods:(1)Stimulate the HL-60 cell lines in vitro with LPS for different time to establish the cellular model of inflammation and simultaneously induce in vivo inflammatoin animal model by LPS.(2)Check the cytotoxic effects of TNF? secretion using MTT colorimetric method for cell proliferaton.(3)Detect the level of expression of TACE carrying out RT-PCR,FCM techniques and immuno-histochemical dying technique.Results:(1)PDQ had the inhibitive effect on TNF?mRNA expression induced by LPS stimulation( P
