Study on DNA Damage Induced by Chlorinated By-Product MX in Drinking Water
- VernacularTitle:饮水氯化消毒副产物——3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮的DNA损伤作用研究
- Author:
Jianjun WU
;
Ailin LIU
;
Wenqing LU
- Publication Type:Journal Article
- Keywords:
Water;
Disinfection;
DNA damage;
Comet assay
- From:
Journal of Environment and Health
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study DNA damage induced by chlorinated drinking water by-product MX.Methods Hu-man hepatocellular carcinoma cell line HepG2cells was selected as target cells to test the DNA damage induced by MX using single cell gel electrophoresis assay.In this study,the cells were treated with MX at final concentrations of10,30,100and300?mol/L.DMSO and hydrogen peroxide were administered as solvent control and positive control respectively.Results MX induced DNA single strand breaks at the concentrations of30,100and300?mol/L.The length of DNA migration induced by MX increased significantly in comparison with the solvent control(DMSO)in a dose-response manner.Conclusions Chlorinated by-product MX in drinking water could induce obvious DNA damage to human hepatocellular carcinoma cell line HepG2cells.
