Cyclosporine A induced toxicity in kidney tubule epithelial cells (NRK-52E)
- VernacularTitle:环孢素A对鼠肾小管上皮细胞的毒性作用
- Author:
Liming WANG
;
Qianying SONG
- Publication Type:Journal Article
- Keywords:
Cyclosporine;
Kidney tubules;
Cytoxieity tests, immnologic
- From:
Chinese Journal of Organ Transplantation
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate and compare cytotoxic effects of cyclosporine A (CsA) treatment in kidney tubule epithelium cells (NRK-52E cells), and whether apoptosis is involved in the CsA-induced toxicity. Methods NRK-52E cells were cultured at 37?℃ in 95?% O 2/5?% CO 2, and were treated with different concentrations of CsA for 48?h.. The damage induced by CsA was characterized by an early cell detachment from culture (by direct counting of adherent cells), lactate dehydrogenase (LDH) release, and the changes in morphology were also observed by a phase-contrast microscope. Assay of apoptosis was done by TUNEL staining. Results CsA treatment induced significant damage in NRK-52E cells, which was demonstrated by obvious signs of cell injury with changes in cell shape, aggregates, and cell loss. A time- and dose-dependent increase in LDH release was also observed for CsA treatment . More than 70?% of the NRK-52E cells treated with 20??g/ml CsA for 48?h were dead. The apoptotic DNA fragments were observed in a population of NRK-52E cells treated with CsA by TUNEL staining, which represented the capacity of CsA to induce apoptosis in NRK-52E cells. Conclusions CsA treatment induced a time- and dose-dependent damage in NRK-52E cells, in which the apoptotic DNA fragments were also observed by TUNEL staining. Thus, the results obtained from the present study suggested that apoptosis, as a possible mechanism, might be involved in the CsA-induced tubulopathy.
