Expression of Nogo-A protein and its significance in the central nervous system of adult mice
- VernacularTitle:Nogo-A蛋白在小鼠中枢神经系统中的表达及其意义
- Author:
Xingxing WANG
;
Xiaoming GUO
;
Linhua YI
- Publication Type:Journal Article
- Keywords:
Spinal cord injuries;
Regeneration, axon;
Myelin shea th;
Axonal growth cone
- From:
Chinese Journal of Trauma
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the cellular distribution of a my elin-associated protein (Nogo-A) in the central nervous system (CNS) of mice a nd explore its possible inhibition on the CNS axon regeneration after spinal cor d injury. Methods Brain, spinal cord, peripheral tissues and w eight-dropping injuried spinal cord from the adult C57BL/6 mice were studied. N ogo-A protein expression was localized immunohistochemically. Chick E12DRG neur ons were cultured and growth cone collapse assessed. Results N ogo-A protein expression detected was mainly in the oligodendrocyte cell body a nd the myelinated axons surrounded by cell processes rather than in the peripher al tissues. After spinal cord injury, Nogo-A was up-regulated at a moderate de gree in the area around the lesion. Chick E12 DRG growth cone collapse rate was as high as 70%, significantly higher than that in the blank control and vector control groups with a significant difference ( P
