Changes of apoptotic features of splenic T lymphocytes in senescent mice
- VernacularTitle:衰老小鼠脾脏T淋巴细胞凋亡特征的变化
- Author:
Zhen WANG
;
Hongbin DENG
;
Diandong LI
- Publication Type:Journal Article
- Keywords:
Apoptosis;
T-Lymphocytes
- From:
Chinese Journal of Geriatrics
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Increased morbidity of auto-immune disease in senescent individual might be related with the defects of apoptosis after stimulation or induction. Changes of apoptotic features in splenic T lymphocytes were studied in senescent mice. Methods Flow cytometry was used to study the rates of apoptosis by analysing sub-G 1 peak. DNA ladder was observed by agarose gel electrophoresis. Free calcium levels in both cells were detected by Fluo-3 loading. Bcl-2 protein levels were detected by Western blot. Results After co-stimulation with IL-2/ConA, flow cytometry showed that average apoptotic percentage of old T cells was 38.3% ?10.3%,significantly lower than that of young ones (58.6% ? 4.0%, P
