Cloning and studying on the expression and function of thyroid hormone-response protein-1 gene, a novel thyroid hormone-response gene from neonatal rat brain
- VernacularTitle:甲状腺激素反应蛋白1基因—一个新的鼠脑甲状腺激素反应基因的克隆、表达分布及其功能预测
- Author:
Chao XIE
;
Min LUO
;
Yisheng YANG
;
Dongsheng CAI
;
Lianxi LI
;
Gang CHEN
;
Wei DING
;
Youping LIU
;
Guo LI
- Publication Type:Journal Article
- Keywords:
Thyroid hormones;
Differential display polymerase chain reaction;
Blot, Northern;
Electronic clone;
Throid hormone-res ponse protein-1 gene
- From:
Chinese Journal of Endocrinology and Metabolism
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To is ol ate novel thyroid hormone-response genes, to study the characterizations of the ir expressions and to predict their possible functions in neonatal rats. Methods A neonatal rat model with congenital hypothyr oidism was established and cDNA fragments of novel thyroid hormone-response gen es from cerebral cortex of neonatal rats were obtained by fluorescence-labeled DD-PCR analysis, subcloning and sequencing. Complete cDNAs of novel thyroid hor mone-response genes were cloned by the techniques of electronic clone, RT-PCR and sequencing, their expressions regulated by thyroid hormone were confirmed b y Northern blot analysis, their distributions, transcription levels in different tissues and different brain areas were further observed by semiquantitative RT -PCR analysis, and their possible functions were postulated through bioinformat ic techniques. Results A novel complete cDNA of thyroid hormone-response protein-1 (TRP-1) gene is cloned. It is 973 bp in f ull-length (Gene Bank accession no. AF348365), and its transcription was enhanc ed in cerebral cortex in neonatal hypothyroidism rats. The expression of its mRN A was very extensive, but more abundant in brain. Its transcriptional level in d ifferent brain areas was not uniform, much higher in olfactory bulb. Its encodin g protein had some significant domains and motifs. Conclusion TRP-1 gene is a new thyroid hormone-response gene and may play an important role during normal brain development. Its abnormal expression may b e partially responsible for neurological defects in brain arising from thyroid h ormone deficiency during critical period for perinatal rats.
