Obtained DC from PBMC recovered from one time used no leukocytes transfusion apparatus
- VernacularTitle:用一次性滤除白细胞输血器回收PBMC制备DC的实验研究
- Author:
Jianwei GUO
;
Liwei QIN
;
Tongde L
- Publication Type:Journal Article
- Keywords:
One time used no leukocytes transfusion apparatus;
PBMC;
DC
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Got high purity dendritic cells from PBMC recovered from one time used no leukocytes transfusion apparatus.Methods:Rinsed PBMC with natural salt water from one time used no leukocytes transfusion apparatus and induced PBMC with GM-CSF and IL-4.Results:Got 5.1?10 7 PBMC from every one time used no leukocytes transfusion apparatus and 4.7?10 6 DCs with a purity of 96.9% were obtained.These DCs express CD86(96.1%)?CD40(88.9%)?CD83(91.6%)?HLA-DR(92.9%)?HLA-ABC(99%)?CD54(97.5%) highly and a few number of these DCs could stimulate proliferation of allogeneic mixed leukocyte reactions strongly.Conclusion:The results of this research supplied basic data for further research of DC and its clinical application.
