Study on mRNA expression of TNFRSF12 in peripheral blood leukocytes of SLE patients
- VernacularTitle:系统性红斑狼疮病人外周血白细胞TNFRSF12mRNA表达的研究
- Author:
Yuan WANG
;
Bo LI
;
Nan SHEN
- Publication Type:Journal Article
- Keywords:
Systemic lupus erythmatosus;
TNFRSF12;
mRNA expression;
TaqMan one-step RT-PCR;
Susceptibility gene
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether the mRNA expression of TNFRSF12 in peripheral blood leukocytes of SLE patients is abnormal and to determine whether the TNFRSF12 gene is susceptibility gene to SLE.Methods:The level of mRNA expression of TNFRSF12 in peripheral blood leukocytes was detected by TaqMan one-step RT-PCR method.Results:The mRNA expression pattern in SLE patients is different to that of normal control.The mRNA in SLE consists of 2 types of isoforms of TNFRSF12,encoding membrane bound glycoprotein and potentially secreted molecules respectively,whereas only the latter in normal control.After PHA treatment,the mRNA expression pattern in normal control becomes the same as that of SLE patients.Overall TNFRSF12 mRNA expression was significantly decreased in active SLE group and significantly increased in lupus nephritis group.Conclusion:The results indicated that TNFRSF12 may play a role in the pathogenesis of SLE and was susceptibility gene to SLE.
