The Imaging Study of Anti-osteosarcoma Monoclonal Antibodies Localized on Human Bone Tumors
- VernacularTitle:抗人成骨肉瘤单克隆抗体对人骨肿瘤定位显像研究
- Author:
Yongyu CHEN
- Publication Type:Journal Article
- Keywords:
bone neoplasmas;
osteosarcoma;
monoclonal antibody, radionuclide imaging
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
For the purpose of improving the accordance rate with early diagnosis of osteosarcoma, we used the osteosarcoma cell line (HOS-8603) to establish 6 cell lines of anti-human osteosarcoma monoclonal antibodies (OSMAb) and sifted out 2 lines (OSMAb-H7, OSMAb-B4) through ELISA and immunofluorescent methods, which could secrete antibodies with high specificity. By antibodies prepared, purified and labeled with 125I we performed a series of experiments in NC nude mouse osteosarcoma models of soft tissue and bone tissue, and found the guiding localization showed stronger specificity. Then we chose OSMAb-B4 treated by bacterial filtration and labeled with 131I to do ?-scintigraphical localization examinations in 8 cases of bone tumor and obtained satisfactory results.
