Relationships between the plasma homocysteine levels and the polymorphisms of itsmetabolic enzymes and the cerebral infarction
- VernacularTitle:血浆同型半胱氨酸水平及其代谢酶基因多态性与脑梗死的关系
- Author:
Chengguo ZHANG
;
Yan SHAO
;
Xueqiang HU
;
Jinhuan CUI
- Publication Type:Journal Article
- Keywords:
Homocysteine;
Oxidoreductases;
Cystathionine beta-synthase;
Polymorphism (genetics);
Brain infarction
- From:
Chinese Journal of Neurology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influences of the genetic factors on the plasma homocysteine (Hcy) level, and the relationships between the plasma homocysteine levels and the polymorphisms in 5,10-methylenetetrahydrofolate reductase (MTHFR) and cystathionine ?-synthase (CBS) and the cerebral infarction. Methods All 87 patients with acute cerebral thrombosis and 80 controls were studied. Plasma Hcy levels were measured by high-performance liquid chromatography-fluorescence detection(HPLC-FD)from using baseline 810 high-performance liquid chromatograph. The presence of the MTHFR C677T mutation was determined by polymerase chain reaction (PCR) assay and subsequent restriction enzyme digestion, and the presence of the CBS G919A or CBS T833C was determined by amplification refractory mutation system. Results Fast plasma Hcy levels were shown higher in the patient group (15.3?4.3) ?mol/L as compared with those in the control group (11.3?3.9) ?mol/L (P
