Cigarette smoking on expression of ICAM-1 in rats' cerebral vascular endothelial cells and volumes of cerebral infarction
- VernacularTitle:烟草烟雾对大鼠脑血管内皮细胞细胞间黏附分子-1表达及脑梗死体积的影响
- Author:
Zhonglan LIU
;
Dayong SUN
;
Hua LI
;
Yu FENG
- Publication Type:Journal Article
- Keywords:
Tobacco smoke pollution;
Endothelium,vascular;
Intercellular adhesion molecule-1;
Brain infarction
- From:
Chinese Journal of Neurology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of different doses and courses of cigarette smoking on the expression of ICAM-1 mRNA, ICAM-1 in rats' cerebral vascular endothelial cells and volumes of cerebral infartion.Methods 105 male Wistar rats(weight 210～220 g) were randomly grouped into long-term massive(20 cigarettes/d,75 d), short-term massive(20 cigarettes/d,30 d), long-term trifle (8 cigarettes/d, 75 d), anti-smoking(20 cigarettes/d, 30 d, denying 45 d)and normal control(no smoking). In situ hybridization, immunohistochemistry were used to determine the expression of ICAM-1 mRNA, ICAM-1 in rat's cerebral vascular endothelial cells (VECs).The VEC's structures were observed by electronic microscope. Left middle cerebral artery occlusion model was established and pathologic image analyzing system were used to determine the ratio of the volume of infarction in hemisphere of the same side. Results On contrast to normal controls, the expression of ICAM-1mRNA, ICAM-1 and the volume of infarction were increased in smoking(P
