Changes of metallothionein contents in rat flap with ischemia -reperfusion injury
- VernacularTitle:缺血再灌注损伤皮瓣组织中金属硫蛋白含量变化
- Author:
Dongyan WANG
;
Jie GUO
;
Dehong GUAN
- Publication Type:Journal Article
- Keywords:
Metallothionein;
Ischemia-reperfusion injury;
Flap
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the changes of metallothionein (M T) in the rat flap with ischemia-reperfusion injury. Methods A ri ght lower abdominal island flap was created in Wistar rats . 16 rats were random ly divided into two groups: the control ( n =8) and the ischemia-reperfusion injury group ( n =8). The contents of MT and malondialdehyde (MDA) were assaye d by 109 Cd-hemoglobin saturation and thiobarbituric acid (TBA) methods. Th e activity of myeloperoxidase (MPO) was measured by colorimetry. Results At ischemia 8 h, reperfusion 12 h and 24 h, the level of MDA in rat i schemia-reperfusion injury group increased 41.7 %, 111.4 %, and 135.7 %, the ac tivity of MPO increased 72.1 %, 218.9 % and 296.0 %, the content of MT increased 42.6 %, 52.8 % and 102.3 %, respectively, compared with the control group ( P
