Expression of zinc finger protein A20 mRNA of mouse liver tissues after trauma with lipopolysaccharide infection
- VernacularTitle:创伤合并内毒素攻击时小鼠肝组织锌指蛋白A20 mRNA表达的初步研究
- Author:
Bo LIU
;
Jianxin JIANG
;
Peifang ZHU
- Publication Type:Journal Article
- Keywords:
Wounds and injuries;
Endotoxin;
Zinc finger protein A20
- From:
Chinese Journal of Trauma
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression law of zinc finger protein A20 mRNA of mouse liver tissues in the process of trauma with lipopolysaccharide (LPS) infection. Methods A total of 95 healthy mice (either sex, from Kunming, Yunnan province) with a mean body weight of 21 g (18-24 g) were randomized into 4 groups: control, trauma only (Group A), LPS only (Group B) and trauma plus LPS (Group C). The models with closed fracture of bilateral spines as well as endotoximia were made. The expression characteristics of zinc finger protein A20 mRNA of liver tissues were measured by reverse transcription-polymerase chain reaction (RT-PCR) and all result data expressed as total gray ratio of A20 mRNA to GAPDH mRNA (?s). Results In the control group, the A20 mRNA expressed at a low level. There was low expression of zinc finger protein A20 in the Group A at various time points, with no significant difference compared with the control group. After LPS infection, the expression of A20 mRNA in the Group B was elevated more obviously than that in the control group at 0.5 hour, reached peak during 0.5-2 hours and decreased after 2 hours. But expression of A20 mRNA at each time point was higher in the Group B than that in the Group A. In the Group C, after LPS infection, the expression of A20 mRNA was elevated more significantly than that in other two groups 0.5 hour, reached the highest level during 0.5-2 hours, much higher than that in the Group B (P
