Clinical study on the expression of lymphocyte activation antigen in peripheral blood of aged patients with non-small cell lung cancer
- VernacularTitle:老年非小细胞肺癌患者外周血T淋巴细胞活化抗原表达的临床研究
- Author:
Qingyong CHEN
;
Jianying ZHOU
;
Yuquan WU
;
Li YAN
;
Ling YANG
;
Zhongyong JIANG
- Publication Type:Journal Article
- Keywords:
Carcinroma, non-small-cell lung;
Antigens,differentiation,T-lymphocyte
- From:
Chinese Journal of Geriatrics
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression of T lymphocyte activation antigen in peripheral blood of aged patients with non-small cell lung cancer . Methods The lymphocytes from peripheral blood in 45 aged patients with non-small cell lung cancer were immunologically labeled in double fluorescence and CD3-FITC, CD25/HLA-DR and CD69-PE and determined by flow cytometry. Normal aged donors, young patiens with lung cancer and aged benign lesion group were used as controls. Results Peripheral blood CD3 +/CD25 +, CD3 +/HLA -DR + and CD3 +/CD69 + in T lymphocyte with 45 aged lung cancer(7.24?1.85,28.46?5.39 and 7.78?2.63, respectively) were significantly lower than those in normal aged controls(10.35?2.54,37.16?5.51,11.02?2.18, respectively)and aged benign lesion (9.53?3.02, 35.33?5.23, 10.67?2.45, respectively)( P 0.05). Significant differences were found among them in stages Ⅲ, Ⅳ(7.15?1.13, 25.32?5.23, 7.14?2.81, respectively) and stagesⅠ,Ⅱ(8.06?1.21, 30.27?6.05, 8.43?2.67, respectively)( P 0.05). Conclusions Detection of CD3 +/CD25 +, CD3 +/HLA -DR + and CD3 +/CD69 + levels by flow cytometry might be helpful for reflecting the human immune function and the prognosis evaluation in patients with aged non-small cell lung cancer.
