Study of the effect of lipofectine-induced antisense oligonucleotide of clusterin on bladder cancer EJ cells
- VernacularTitle:脂质体介导的聚集素反义寡核苷酸对膀胱癌EJ细胞的作用
- Author:
Lili LIANG
;
Qiang ZHANG
;
Chong LEE
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Carcinoma;
Clusterin
- From:
Chinese Journal of Urology
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of clusterin on the cytobiological features of bladder cancer EJ cells. Methods EJ cells were transfected using lipofectine-induced antisense oligonucleotide of clusterin,and their expression was blocked.The biological behaviors of EJ cells,such as proliferation,apoptosis and necrosis,were observed. Results Compared with the sense group which were transfected by sense oligonucleotide of clusterin,the antisense group which were transfected by antisense oligonucleotide of clusterin exprerienced increase in apoptosis of EJ cells,reaching the peak on day 5 with apoptosis rate of 80%.The difference between the 2 groups was statistically significant ( P
