Expression of dihydrodiol dehydrogenase 2 in prostate cancer and its significance
- VernacularTitle:二氢二醇脱氢酶2在前列腺癌中的表达及意义
- Author:
Weidong SONG
;
Liqun ZHOU
;
Lili LIANG
- Publication Type:Journal Article
- Keywords:
Prostate neoplasms;
Carcinoma;
Dihydrodiol dehydrogenase
- From:
Chinese Journal of Urology
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of dihydrodiol dehydrogenase (DD) in prostate cancer and its significance. Methods With reference to expression of ?-actin gene,the expression level of a human dihydrodiol dehydrogenase isoform (DD2) mRNA was examined in prostate cancer tissues (11 cases) and normal prostate tissues (10 cases) by reverse transcriptive-polymerase chain reaction(RT-PCR). Quantitative determination of relevant band densities was performed using densitometry-scanning techniques. Results Strong expression of DD2 mRNA was detected in prostate cancer tissures with absorbance in the range of 0.550 to 1.018 (median,0.726),and low expression of DD2 mRNA in normal prostate tissues with absorbance in the range of 0.248 to 0.420 (median,0.333). The difference of the expression of DD2 mRNA between cancer and normal prostate was significant ( P
