Mutation of rspL gene of Mycobacterium tuberculosis in urine and its relationship with streptomycin-resistance
- VernacularTitle:尿液中结核杆菌rspL基因突变与其耐药性关系的研究
- Author:
Li WANG
;
Jiangtao ZHANG
;
Meirong ZHAO
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Gene mutation;
Streptomycin;
Drug resistance
- From:
Chinese Journal of Urology
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the streptomycin(SM)-resistance of Mycobacterium tuberculosis (MTB) in urine and to examine their relationships with the mutation of rspL gene. Methods 47 strains of MTB were isolated from the urine of patients with renal tuberculosis.The mutation of rspL gene were analyzed by PCR-SSCP with silver-staining,and the strains were tested for the sensitivity to streptomycin. Results All the 47 strains had positive expression of rspL gene of MTB,with 28 SM-resistant and 19 SM-sensitive.Among the 28 SM-resistence strains 19(68%) had rspL gene mutation by SSCP testing.The 19 strains of SM-sensitive had no mutation. Conclusions Detecting mutation of MTB rspL gene in urine by PCR-SSCP can rapidly and exactly identify the MTB's sensitivity to streptomycin,which is significantly important in clinical treatment of anti-tuberculosis.
