Exploration of the receptor of Schwann cell derived neurotrophic factor in rat peripheral nerve
- VernacularTitle:许旺细胞源神经营养因子受体在外周神经组织中的分布研究
- Author:
Shaoan CHENG
;
Jiakai ZHU
- Publication Type:Journal Article
- Keywords:
Schwann cell;
Neurotrophic factor;
Receptor;
Peripheral nerve
- From:
Chinese Journal of Microsurgery
2000;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whetherthere exists Schwann-cell derived neurotrophic factor (SDNF) receptor in peripheral nerve. Methods SDNF binding sites in rat sciatic nerve were studied using 125 Ⅰ-SDNF as a radioligand (radioligand binding assays). Results There exists SDNF specific binding sites in peripheral nerve,and the specific binding sites have the following characteristics:①The equilibrium dissociation constant (kd) was (93.11?0.52) pmol/L.②The maximal binding capacity (B max ) of SDNF was (8.91?0.26)fmol/mg protein.③Saturation.④Kinetic studies revealed that the association rate constant (K 1) was (3.91?0.63)?10 7M -1 ?min -1 and the dissociation constant (K -1 ) was (3.38?0.54)?10 -3 min -1 .⑤Specific studies of SDNF binding sites showed that the binding sites were highly specific for SDNF. Conclusion SDNF receptor exists in the peripheral nerve and the peripheral nerve may be one of target tissues of SDNF.
