APS IgG stimulates monocytes to express TF activity,not via Fc receptor pathway
- VernacularTitle:APS IgG通过非Fc受体途径诱导血液单核细胞表达TF活性
- Author:
Hong ZHOU
;
Junxia GU
;
Wenrong XU
- Publication Type:Journal Article
- Keywords:
Antiphospholipid syndrome;
Autoantibodies;
Monocytes;
Tissue factor
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether the IgG from patients with antiphospholipid syndrome(APS) is capable of stimulating blood monocytes to express tissue factor(TF) activity and to explore the mechanisms possibly concerned.Methods:Freshly isolated peripheral blood monocytes were cultured in media containing purified APS IgG or normal IgG or RP-1 [a monoclonal anti-?_2 glucoprotein I(?-?_2GPI)antibody] and/or other agonists and the TF activity on monocytes was investigated by measuring factor Ⅶa-dependent generation of factor Xa.Results:APS IgG as well as RP-1 significantly increased monocytes TF activity,comparing to normal IgG;exogenous ?_2GPI enhanced the effects as the intact IgG molecule.Conclusion:That certain antiphospholipid antibodies,mainly anti-?_2GPI antibodies,induce monocytes TF activity in an antigen-specific manner,not via Fc receptor pathway,is contributed to the thrombotic diathesis in APS.
