Differential expression of Rac2 and RhoGDI in the neutrophil and its role in coronary heart disease
- VernacularTitle:中性粒细胞小GTP结合蛋白的表达与冠心病发生关系的研究
- Author:
Yanling LIU
;
Yan XIONG
;
Jianmin WU
- Publication Type:Journal Article
- Keywords:
Neutrophil;
Reactive oxygen species;
Rac2;
RhoGDI
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Evaluate the role of reactive oxygen species production and the differential expression of Rac2 and RhoGDI of neutrophils in pathogenesis of coronary heart disease.Methods:Reactive oxygen species production was measured by chemiluminescence method,the mRNA expression of Rac2 and RhoGDI was detected by quantitative RT-PCR.Results:The reactive oxygen species production by neutrophil is significantly increased in the coronary heart disease group,and the increase is related to the inflammatory cytokines in plasma.There is a significant increase of Rac2mRNA expression in coronary heart disease group than that of the control,while the expression of RhoGDImRNA in two groups are not significantly different.The ratio of Rac2mRNA to RhoGDImRNA is correlated to the production of reactive oxygen species of neutrophils.Conclusion:Netrophils are involved in the pathogenesis of coronary heart disease through their reactive oxygen species production,and the abnormal expression of Rac2 and RhoGDI is an important cause leading to the increased reactive oxygen species production.
