Effects of ultrasonic cavitation on cultured cell membrane in vitro
- VernacularTitle:超声波空化效应对体外培养细胞细胞膜作用的实验研究
- Author:
Haitao RAN
;
Hong REN
;
Zhigang WANG
- Publication Type:Journal Article
- Keywords:
Ultrasonics;
Cells,cultured;
Cell membrane;
Cavitation
- From:Chinese Journal of Ultrasonography
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of ult rasonic cavitation on cell membrane cultured in vitro. Methods The rat pulmonary artery vascular smooth muscle cells were divided into three groups, control group, simple ultrasound radiation group and contrast plus ultrasound radiation group.The latter two groups were exposed to ultrasound(665 kHz, 0.5- W/cm 2) for 20 s but the last group with contrast agent. Immediately after the experiment or 24 hours after treatments, electron microscopic scanning was used to observe the membrane change. Results Compared with control group, the cell structure and cell surface had no apparent changes in the second group treated with ultrasound alone. In the third group, nearly 20% cells exhibited small holes with diameter mostly about 1-2 ?m in the cell surface and returned to normal appearance after 24 hours later. Conclusions Ultrasonic cavitation can cause small transient holes in cell surface and hence increase permeability of the cell membrane. It might be the main mechanism for gene delivery with ultrasound contrast agents.
