Effect of antisense VEGF gene on VEGF expression and growth of human renal cell carcinoma
- VernacularTitle:人反义血管内皮生长因子基因表达对肾癌细胞的影响
- Author:
Dongkui SONG
;
Zhouhui PAN
;
Taisen YANG
- Publication Type:Journal Article
- Keywords:
Kidney neoplasms;
Carcinoma;
Blood vessels;
Endothelium;
Endothelial growth factor
- From:
Chinese Journal of Urology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of human antisense VEGF gene on VEGF expression and growth of renal cell carcinoma Methods Reverse transcription-polymerase chain reaction (RT-PCR) for VEGF,VEGF was inserted into eukaryotic expression vector pcDNA3.1 to construct eukaryotic expression vector carrying human VEGF cDNA,then using restrict enzyme to confirm the result.The vector was transfected into renal cell carcinoma 780-0 and positive clone was selected by using G418.VEGF expression was detected by using immunocytochemical technique and the growth curve was detected by using MTT method. Results VEGF gene was gained by RT-PCR and antisense VEGF eukaryotic expression vector pcDNA 3.1 was constructed.The positive cell rate of VEGF expression in pcDNA 3.1-(antisense)VEGF group( 10.3%) is lower than that in 780-0-PC group(92.8%) or 780-0 group(96.6%), P
