Level of granzyme B mRNA and perforin mRNA in urine and acute rejection of the transplanted kidney
- VernacularTitle:尿穿孔素和粒酶Bm RNA检测在诊断移植肾急性排斥反应中的意义
- Author:
Bo ZHANG
;
He WANG
;
Geng ZHANG
- Publication Type:Journal Article
- Keywords:
Kidney;
Graft rejection
- From:
Chinese Journal of Urology
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the level of granzyme B mRNA and perforin mRNA in urine on the diagnosis of renal acute rejection. Methods A total of 34 cases of renal transplantation were studied.The urine samples were measured with the use of a competitive and quantitative polymerase-chain-reaction assay for granzyme B mRNA and perforin mRNA and the data were analysed by using the SPLM statistical software,mRNA levels were log-transformed. Results The levels of granzyme B mRNA and perforin mRNA in urine were higher in patients with acute rejection than in patients without acute rejection.The level of perforin mRNA in patients with acute rejection was (1.2?0.4)fg/?g of total RNA and in patients without acute rejection was (-0.6?0.3)fg/?g of total RNA (P
