Cloning and analyzing of interleukin-4 cDNA from the lymphocyte of Tibet pig
- VernacularTitle:藏猪白细胞介素4基因cDNA的克隆及序列分析
- Author:
Hui LI
;
Rong GAO
;
Mei WU
- Publication Type:Journal Article
- Keywords:
Tibet pig;
Interleukin 4 gene;
Cloning;
Sequence analysis
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone interleukin 4 cDNA of Tibet pig.Methods:To amplify cDNA,using RT PCR from the total RNA from the lymphocyte stimulated by 10 ?g/ml Con A in vitro,to subclone into pMD T vector,and then to determine it by digesting and sequencing.Results:The cloned Tibet pig interleukin 4 was 98% homologus to the necleotide acids of Chenghua pig a special specie of pig in Sichuan province and 99% homologus to that of hybrid of Landrance with Chanbai pig.Conclusion:IL 4 of Tibet pig was successfully cloned from the lymphocyte after stimulating 70 h with ConA.
