FasL-cDNA transfected into mouse hematopoietic cells to prevent graft versus host disease
- VernacularTitle:转染mFasL-cDNA小鼠造血细胞预防移植物抗宿主病的实验研究
- Author:
Zhiliang XU
;
Ping ZOU
;
Lingbo LIU
- Publication Type:Journal Article
- Keywords:
Fas Antigens;
Ligands;
Gene transfer;
Bone marrow transplantation
- From:
Chinese Journal of Organ Transplantation
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the new method to prevent graft versus host disease (GVHD) by clearing T lymphocytes of bone marrow graft through Fas-FasL way. Methods FasL-cDNA was transfected into BALB/C mouse hematopoietic cells by liposomes. The transfected cells were cultured together with BAC mice bone marrow graft. The mixed bone marrow graft was injected into BALB/C mouse recipients after 60 Co-? ray irradiating. Then the mortality, manifestation and pathologic change of GVHD of recipient mice were observed. Results Compared with control group, the mortality of the recipients in the experimental group was markedly decreased in 60 days (20% vs 70%, P
