Effects of 1,25-dihydroxyvitamin D_3 on T-lymphocyte subsets,mixed lymphocyte reaction and natural killer cell following skin transplantation in mice
- VernacularTitle:1,25-(OH)_2D_3对小鼠皮肤移植后脾T细胞亚群、MLR及NK细胞活性的影响
- Author:
Jinsheng ZHANG
;
Delin GUAN
;
Jianjun XU
- Publication Type:Journal Article
- Keywords:
1,25-dihydroxyvitamin D_3;
Immunosuppression;
Skin transplantation;
T lymphocyte
- From:
Chinese Journal of Organ Transplantation
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the mechanism of immunosuppressive activity of 1,25-dihydroxyvitamin D_3 and provide a theoretical basis for clinical use. Methods Different inbred strain male BALB/C (H-2d) and male C57BL/6(H-2b) mice were used as skin transplantation donors and recipients, respectively. After operation C57BL/6 mice were conditioned with 1,25-dihydroxyvitamin D_3 2.5 ??g/kg every day, Cyclosporine A (CsA) po 25 mg/kg every day separately or unitedly. Ten days after transplantation, the recipients were sacrificed, and the spleens were collected. The mouse splenic T lymphocytic subsets, mixed lymphocyte reaction (MLR), the activity of natural killer (NK) cells were determined. Results The mean survival time (MST) of skin allografts was prolonged from ( 9.75 ? 0.89 ) days to ( 13.13 ? 1.13 ) days by treatment of the recipient mice with 1,25-dihydroxyvitamin D_3. CD3 + and CD4 + subset percentage in 1,25-dihydroxyvitamin D_3 group was lower than that in control group CD3 + ( 40.19 ? 4.25 )% vs ( 48.70 ? 7.19 )%, P
