Effect of adenovirus-mediated bcl-2 transfer on regeneration and repair of peripheral nerves in rats
- VernacularTitle:腺病毒载体介导bcl-2基因转染对周围神经再生修复的影响
- Author:
Ping YANG
;
Dajun YING
;
Lin SONG
- Publication Type:Journal Article
- Keywords:
Peripheral nerves;
Nerve regeneration;
Genetic vectors;
bcl-2
- From:
Chinese Journal of Trauma
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of adenovirus-mediated bcl-2 transfer on the regeneration and repair of peripheral nerves in rats. Methods The sciatic nerve-transected model was selected and sutured end to end with epineurium. Then, adenovirus/sense-B-lymphoma/leukemia-2 (Ad/s-bcl-2), adenovirus/anti-sense-B-lymphoma/leukemia-2 (Ad/as-bcl-2), adenovirus/?-galactosidase (Ad/LacZ) and normal saline (NS) were injected into the sciatic nerve 0.5 cm away from the sutured point respectively. The rats were perfused with 4% paraformaldehyde at the 48th hour, 7th, 15th and 30th days respectively after operation. The spinal cords L 4-6 were harvested, frozen sectioned and given x-gal staining, bcl-2 in situ hybrid and immunohistochemical staining and GAP-43 immunohistochemical staining. The postoperative functional recovery of the rear limbs of rats was dynamically observed by using sciatic nerve functional index (SFI). Results The GAP-43 expression in the motor neurons of anterior horn of spinal cord in group Ad/s-bcl-2 was higher than that in other groups (P0.05). Conclusions Gene transfer of bcl-2 can promote regeneration and functional recovery of the peripheral nerves following sciatic nerve injury.
