The effect on cardiac function of autologous smooth muscle cells transplant into ischemic myocardium of rat and observation of its long-lerm survival
- VernacularTitle:大鼠自体平滑肌细胞移植治疗心肌缺血的长期效果观察
- Author:
Ming LIU
;
Baoren ZHANG
;
Liangjian ZOU
;
Jialin ZHU
- Publication Type:Journal Article
- Keywords:
Transplantation;
Myocardial ischemia
- From:
Chinese Journal of Geriatrics
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The long-term effects of cell transplant on cardiac function are unknown. Therefore, we tested the survival and functional impact of rat autologous smooth muscle cells up to 12 weeks after transplant into infracted hearts. Methods Autologous smooth muscle cells were acquired from the ductus deferens of adult Sprague-Dawley rats(weight,300g), and cultured for 4 weeks before transplant. 4 weeks after left coronary artery ligation, the cultured cells(4?10 6 , n=10), marked with 5-bromo-2'-deoxyuridine(BrdU), or culture medium alone(n=10) were injected directly into infarcts of the heart. Cardiac function was assessed by echocardiography before and 12 weeks after transplantation. BrdU in the cells and smooth muscle cells were identified by immunohistochemical staining technique using monoclonal antibodies against BrdU and ?-smooth muscle actin. Results Grafted autologous smooth muscle cells were presented in infarcts and formed musclelike tissue. They thickened the wall of the left ventricle〔(2.36?0.31) vs (1.03?0.11)mm,P
