Interferon gamma enhances the cytotoxicity of 5′-deoxy-5-fluorouridine and 5-fluorouracil against human bladder cancer cells
- VernacularTitle:干扰素?增加去氧氟尿苷和5-氟尿嘧啶抗膀胱癌作用的实验研究
- Author:
Gang LI
;
Yuanfang ZHANG
;
Qiang DING
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
Drug therapy;
Endothelial growth factors
- From:
Chinese Journal of Urology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulative effect of interferon gamma (INF?) on the expression of platelet-derived endothelial cell growth factor / thymidine phosphorylase (PD-ECGF/TP) and its relation with the anti-cancer effect of 5′-deoxy-5-fluorouridine (5'DFUR) and 5-fluorouracil (5FU) in T24 bladder cancer cells. Methods PD-ECGF/TP mRNA and protein expressions were determined by RT-PCR and Western blot,respectively.Cytotoxicity of 5′DFUR,5FU or mitomycin C (MMC) against T24 cells was evaluated by MTS assay. Results The expressions of PD-ECGF/TP mRNA and protein were concomitantly elevated in T24 cells after INF? treatment.And INF? decreased IC 50 s of 5′DFUR [from (16.0? 3.6 )mmol/L to (6.2?0.9)mmol/L,( P
