Resistin gene overexpression in adipose tissue of SD rats with high-fat diet and its correlation with leptin and insulin
- VernacularTitle:高脂大鼠抵抗素基因的上调表达及与胰岛素、瘦素的相关性
- Author:
Shaoyun WANG
;
Huaidong SONG
;
Rongying LI
;
Jinfeng TANG
;
Fengying LI
;
Libin ZHOU
;
Ying YANG
;
Renming HU
;
Bo MOU
;
Mingdao CHEN
- Publication Type:Journal Article
- Keywords:
Resistin;
Gene expression;
Insulin;
Leptin
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe expression of resistin gene in adipose tissue of SD rats fed with high-fat diet and its correlation to insulin resistance, diabetes mellitus and leptin. Methods Using RT-PCR to mRNA from adipose tissue, the products were obtained, purified, constructed and squenced. Once its squence was verified, semi-quantitative RT-PCR and Northern Blot were employed to study its expressions in adipose tissues of rats with different diets. Oral glucose tolerance test and insulin release test were performed in 2 groups of rats. Results (1) The resistin gene was successfully constructed and sequenced, which was in accordance with the data of genbank. (2) Compared with the control, the expression of resistin in adipose tissue of high-fat diet rats was significantly enhanced, and blood glucose, serum insulin and leptin levels were also elevated in high-fat diet rats (P
