Identification of difference of gene expression in the visceral adipose tissue of the lean type 2 diabetic rats
- VernacularTitle:非肥胖2型糖尿病大鼠腹腔脂肪组织基因差异表达的鉴定
- Author:
Jialin YANG
;
Guo LU
;
Fanglin ZHANG
;
Youpin LIU
;
Di ZHANG
;
Wenzhong ZHOU
;
Guanwu XU
;
Yisheng YANG
;
Min LUO
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus, experimental;
Rats, Sprague-Dawley;
Gene expression profiling;
Expressed sequence tags
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the difference of gene expression in the visceral adipose tissue of the lean type 2 diabetic rats. Methods The difference bands were generated by the representational difference analysis ( cDNA RDA) ; the final difference products was ligated into the pUC-18 vector, subcloned and sequenced, and the obtained expressed sequence tags ( ESTs) were given bioinformatics analysis, and then the expression level of known gene was verified by semi-quantitative RT-PCR preliminarily. Results Six novel ESTs and 1 known gene [ rat lipoprotein lipase ( LPL) gene) were obtained in visceral adipose tissue of rat. One of the ESTs was partially similar to mouse musculus ERA-like GTPase (Era) gene. LPL gene was up-regulated in the visceral adipose tissue of the lean type 2 diabetic rats and those fed with fat-enriched food. Conclusion Six novel ESTs and 1 known gene in high expression are obtained from visceral adipose tissue of rat. LPL gene among them is up-regulated and may be related to the high fat and high calorie diet, hyperlipidemia, insulin resistance and molecular pathogenesis of the lean type 2 diabetic rat.
