Effect of leptin on protecting rat islet ?-cells against lipotoxicity induced by free fatty acid
- VernacularTitle:瘦素对游离脂肪酸致大鼠胰岛?细胞脂毒性的保护作用
- Author:
Yu TONG
;
Xiaohui GUO
;
Yan GAO
- Publication Type:Journal Article
- Keywords:
Islets of Langerhans;
Leptin;
Fatty acids, nonesterified;
Lipotoxicity
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether leptin protects rat islet ?-cells from lipotoxicity induced by free Taity acid ( FFA). Methods Pancreatic islets were isolated from Wistar rats. Isolated islets were divided into 4 groups; ( 1 ) Control group; (2) Leptin group (murine recombinanl leptin 100?g/L) ; (3) FFA group [0. 25 mmol/L long chain fatty acid (oleate: palmitate =2: 1 ) ] ; (4) FFA + Leptin group (both leptin and FFA were added to the culture medium). Alter being cultured lor 72 hours, triglyceride ( TG) content in islets, insulin secreted under basal and glucose stimulated conditions, and the mRNA expression of fatty acid oxidase-carnitine palmityl transferase- 1 ( CFT- I ) , fatty acid synthetase-acyl-CoA carboxylase ( ACC) , peroxisome proliferator activated receptors ( PPAR)a and PPARr in islets were examined. Results ( 1 ) TC content in islets was elevated in FFA group as compared with control group ( P
