The role of transcription factor NF-kB in the INS-1 cell impairment induced by IL-1? and other cytokines
- VernacularTitle:核转录因子NF-?B在IL-1?及其他细胞因子介导的INS-1细胞损害中的作用
- Author:
Liyong YANG
;
Yongnian YANG
- Publication Type:Journal Article
- Keywords:
NF-kB;
Interleukin-1;
Tumor necrosis factor;
Interferon type Ⅱ;
Islets of langerhans
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of NF-kB in cytokines (IL-1?, TNF-? and IFN-r) -induced impairment of INS-1 cell, a pancreatic islet ?-cell line. Methods Retrovirus expressing IkB??N with mutant IkB?(inhibitor of NF-kB ) was constructed. Immunoblot analysis, fluorescence analysis using a kB-1uc reporter gene and thiazolyl blue viability assay were applied in this study. Results IL-1?-induced IkB? degradation and NF-kB activation were found in INS-1 cells but not in INS-1/IkB??N cells infected with IkB??N retrovirus. The viability of INS-1 cells incubated with the combination of IL-1? and IFN-r or TNF-? and IFN-r was decreased but INS-1/IkB??N cells could resist these cytokines-induced decrease of cell viability. Conclusion IL-1?, TNF-?can decrease the viability of? cells, while IFN-r may have some enhancing role. Inhibition of NF-kB activation can protect ? cells from the cytotoxicity of the cytokines.
