Association of oxidative stress inducing neuronal apoptosis with c-Myc,Fas-FasL and nuclear factor-?B
- VernacularTitle:氧化应激诱导神经细胞凋亡与c-Myc、Fas-FasL、核因子-?B关系的研究
- Author:
Rutong YU
;
Xiang LI
;
Lida GAO
- Publication Type:Journal Article
- Keywords:
Neurons;
Proto-oncogene protein, c-Myc;
Fas-FasL;
Nuclear factor-?B;
Apoptosis
- From:
Chinese Journal of Trauma
1993;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between oxidative stress inducing neuronal apoptosis and the protein expressions of c-Myc, Fas-FasL and nuclear factor ?B (NF-?B) in neurons. Methods The primarily cultured neurons of SD rat in vitro were divided into 5 groups: Group A (control), Group B (treated with hypoxia), Group C (treated with small dose of H 2O 2), Group D (treated with hypoxia and SOD) and Group E (treated with H 2O 2 and SOD). Then, the neuronal apoptosis was elevated with TUNEL,Gel electrophoresis and flow cytometry. The protein expressions of c-Myc, Fas-FasL and NF-?B were detected with immunohistochemistry. Results Apoptosis rates of the Groups B and C were 6 and 8 times than that of the Group A respectively ( P
