The proliferation effect of sodium orthovanadate on osteoblast cell line MC3T3-E1 and the correlation between the effect and nitric oxide concentration
- VernacularTitle:原钒酸钠对成骨细胞株MC3T3-E1增殖的影响及其与一氧化氮浓度的关系
- Author:
Xiaoying SHEN
;
Aizhen ZHANG
;
Jie YAN
- Publication Type:Journal Article
- Keywords:
Vanadates;
Nitric oxide;
Osteoblasts
- From:
Chinese Journal of Geriatrics
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the proliferation promoting effect on osteoblast cells induced by sodium orthovanadate and whether nitric oxide (NO) was involved in this process. Methods MTT assay was employed to determine cell proliferation and its inhibition. The level of NO was measured by enzyme reduction method. Results The MTT values of MC3T3-E1 cells under the 2.5,5.0,10.0 ?mol/L of sodium orthovanadate were 0.3380?0.0045, 0.3400?0.0141, 0.3840?0.0313 respectively, which were higher than in control group (0.2540?0.0167)(P
