Cyclooxygenase-2 expression and its localization in the mature rat testis
- VernacularTitle:环氧化酶-2在正常成年大鼠睾丸中表达的研究
- Author:
Xiaohui LIU
;
Zhiwen ZHANG
;
Xiuying LIANG
- Publication Type:Journal Article
- Keywords:
Infertility,male;
Cyclooxygenase-2;
Rats
- From:
Chinese Journal of Urology
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To confirm the expression and localization of COX-2 gene in mature rat testis,as COX-2 not only plays a central role in inflammation by up-regulating the synthesis of prostaglandins,but also mediates critical reproductive functions by other mechanisms. Methods In the mature Wistar rats,COX-2 expression and its localization were examined by using immunohistochemistry,RT-PCR and Western-blot. Results The results of RT-PCR and Western-blot showed that COX-2 mRNA and its protein exist in the testis. Expression of COX-2 was localized in spermatogenic cells on immunohistochemical study.No COX-2 expression in sertoli cells and leydig cells was found. Conclusions The present study confirmed the expression of COX-2 in rat testis and its localization in spermatogenic cells.These suggest that COX-2 may play a role in spermatogenesis.
