Effect of integrin expression on procollagen synthesis of fibroblasts in wound healing
- VernacularTitle:整合素表达对伤口愈合时成纤维细胞合成原胶原的影响
- Author:
Minliang CHEN
;
Jiake CHAI
;
Zihao LIN
- Publication Type:Journal Article
- Keywords:
Wound healing;
Fibroblasts;
Tropocollagen
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of integrin expression on procollagen synthesis of fibroblasts in wound healing. Methods Antisense oligonucleotides were used to intercept the expression of integrin ? 5 or ? 1 subunit on fibroblasts respectively in wound healing, then the changes of procollagen mRNA were detected by RT-PCR. Results The antisense oligoncleotdes could specifically inhibit the expression of integrin subunit. Decreased expression of integrin?5 or ?1 subunit on fibroblasts significantly lowered mRNA of procollagen ? 1(Ⅰ) and ? 1(Ⅲ). Conclusion Reduction of integrin expression on fibroblasts in wound may inhibit procollagen synthesis in the level of transcription. These results provide the experimental gists on studying of scar reduction in wound healing.
