Tooth development in vitro cultured mouse mandibular arch
- VernacularTitle:小鼠牙胚发育在下颌弓培养模型中的动态观察
- Author:
Shisen LIU
;
Buling WU
;
Qing YU
- Publication Type:Journal Article
- Keywords:
Organ culture;
Tooth germ;
Odontogenesis;
Trowell organ culture system
- From:
Journal of Practical Stomatology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a model for the study of tooth initiation and early development by in vitro culture of mouse mandibular arch . Methods:42 mandibular arches of 11dpc fatal mouse were dissected and cultured in Trowell organ culture system for 11 days.Regular histologic observation was performed to observe the initiation and development of tooth.Results: Mandibular arches grew well in Trowell culture system.The initiation and early development of tooth was found.The explants maintained to cap stage.On day 11,necrosis of the cultured mandibular arches was observed and the culture was ended. Conclusion:In vitro cultured mandibular arch can be used as a model for the study of tooth initiation and early development.
