Establishment and characterization of a metastatic cell line from spinal cord metastasis induced by injection of Mc3 cells in nude mice
- VernacularTitle:裸鼠脊髓转移灶粘液表皮样癌细胞系Ms的建立及生物学特性
- Author:
Honglin YANG
;
Junzheng WU
;
Bin LIU
- Publication Type:Journal Article
- Keywords:
Salivary glands;
Mucoepidermoid carcinoma;
Neoplasm metastasis;
Sub-cellline
- From:
Journal of Practical Stomatology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a metastatic cell line from distant organ metastasis using Mc3 cell line in nude mice. Methods: Tail vein injection of Mc3 cells and cell culture technic were employed to induce metastasis in distant organ . Cell counting and flow cytometry were used to study the cell growth. Karyotype analysis and histopathological observation were used to study the morphological features with light and electron microscopy. Results: Paralized nude mouse was observed in 1 out of 50 experimental nude mice. The cells derived from the spinal cord were cultured and transferred for more than 50 passages. The cells were proved to be of mucoepidermoid carcinoma from human being by the morphology, histopathology and karyotype of the cells. The population doubling time and S-phase cell of the cells were 43 h and 22.7% respectively. The cell line was named Ms. Conclusion: Ms is a metastatic cell line of spinal cord metastasis in nude mouse derived from human mucoepidermoid cacinoma cells.
