Experimental study of using induced bone marrow stromal cells into a tissue-engineered bioartificial nerve
- VernacularTitle:经诱导的骨髓基质干细胞应用于组织工程化人工神经的实验研究
- Author:
Saiyun HOU
;
Jiakai ZHU
- Publication Type:Journal Article
- Keywords:
Bone marrow stromal cells;
Schwann cell;
Nerve graft;
Tissue engineering
- From:
Chinese Journal of Microsurgery
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effectiveness of using induced bone marrow stromal cells into a tissue-engineered bioartificial nerve on bridging a 10mm long sciatic nerve defect Methods Twenty-eight F344 female rats weighing 160～200 g were randomly divided into four groups of nerve grafting,with 7 rats in each group A group:PLGA tubes with an intrinsic framework were seeded with syngeneic bone marrow stromal cells induced 5 days;B group:PLGA tubes with an intrinsic framework were seeded with syngeneic Schwann cells;C group:PLGA tubes were only filled with an intrinsic framework;D group:autografts Three months later,a series of examinations were performed,including:electrophysiological methods,Walking trace's analysis,hisotological staining of nerves,S-100 and neurofilament immunostaining and axon counts Results At 12 weeks after the operations,all the examinations of A group were better than C group( P 0.05). Conclusions Induced bone marrow stromal cells as seeding cells for peripheral nerve tissue engineering were used to repair 10mm nerve gap,the outcome was satisfied.
