The inhibition of IL-1? and IL-6 release in LPS induced Hela cells by cPLA_2 antisense oligonucleotide
- VernacularTitle:转染反义磷脂酶A_2寡核苷酸抑制LPS诱导Hela细胞分泌IL-1?和IL-6
- Author:
Xiaohui WANG
;
Guangtao YAN
;
Xiuhua HAO
- Publication Type:Journal Article
- Keywords:
cPLA 2;
LPS;
Antisense oligonucleotide;
IL-1?;
IL-6
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of cPLA 2(cytosolic PLA 2, 85-kD PLA 2) on the signaling pathways of IL-1?、IL-6 release in LPS induced Hela cells.Methods:①LPS stimulated Hela cells were exposed to the complex of cPLA 2 initiation site-directed antisense oligonucleotide and liposome in the indicated doses and time, and then,analyzed the changes of cPLA 2 both in mRNA and in protein by RT-PCR and Western blot.②At the same time, the supernatants were collected to detect the level of IL-1? and IL-6 under radioimmunoassay(RIA).Results:①The transfection led to a dose-dependent decrease in cPLA 2 protein, while the change of mRNA is not notable.②The levels of IL-1?、IL-6 in the transfected cells were reduced following the repression of cPLA 2 compared with that of LPS added only.Conclusion:cPLA 2 probably plays an important role in the signaling mechanisms on IL-1?、IL-6 release in LPS-induced Hela cell. [
