Separative Purification of and Characteristic Research on Human Blood Type Substance P1
- VernacularTitle:人血型物质P_1的分离纯化及性质研究
- Author:
Yunfang WANG
- Publication Type:Journal Article
- Keywords:
Blood type, substance P1, glucoprotein.
- From:
Chinese Journal of Blood Transfusion
1988;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
A Human blood type antigen P1 with very high activity was separated from sheep hydatid cyst fluid. Determination of the antigen by ultraviolet spectrum, polyacrylamide electrophoresis, gel filtration, and determination of its carbohydrate content and aminoacid residue had indicated that the substance was a glycoprotein, its total carbohydrate content was 80％ about one-fifth of which was hexosamine, and other carbohydrate components were glucqse, galactose and glucosamine. Its protein consisted of 14 aminoacids, and its molecular weight was about 30000 Daltons. By immunizing the animals with this substance, the anti-P1 serum of high titer could be obtained. The successful extraction of this substance P1 with high activity would provide the abundant resource for reseach on human blood type antigen and its immunochemistry, and create the good conditions for studies in preparation and application of anti-P1 serum or monoclonal antibody toward P1.
