Effects of tumor necrosis factor alpha(TNF-?) on the fungicidal activity of mouse peritoneal exudate cells
- VernacularTitle:TNF-?对小鼠腹腔渗出细胞抗真菌活性的影响
- Author:
Tiantuo ZHANG
;
Yuqi ZHOU
;
Fengli ZHOU
- Publication Type:Journal Article
- Keywords:
TNF-?;
Fungicidal activity;
Peritoneal exudate cells
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of TNF-? on the activation and fungicidal activity of mouse peritoneal exudate cells.Methods:The mouse peritoneal exudate cells and macrophage were stimulated with IL-12、IL-18、IFN-?、TNF-? or monoclonal antibody.Cytokines and nitric oxide(NO) levels in the medium supernatant were determined with ELISA and Griess method respectively.Detected the numbers of viable Cryptococcus neoformans in macrophage.Results:Combined with IL-12 and IL-18 synergistically induced the production of TNF-? by peritoneal exudate cells and this effect can be inhibited by neutralizing anti-IFN-? mAb.Combined with IFN-? and TNF-? synergistically induced the production of NO by macrophage and potentiate its activity against Cryptococcus neoformans.Conclusion:The result suggests that the TNF-? plays an important role in enhancement of the fungicidal activity of macrophages. [
