The study of biological activity of the expression products of the human ?-NGF cDNA in CHO cell line and purified the seperated protein
- VernacularTitle:人?-NGF基因在CHO细胞中表达的生物学活性及分离纯化
- Author:
Yanyan BAI
;
Jicheng YANG
;
Jingcheng MIAO
- Publication Type:Journal Article
- Keywords:
Human ?-NGF;
Transfection of eukaryotic cell;
Expression
- From:
Chinese Journal of Immunology
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the biological activity of the expression products of the human ?-NGF cDNA in CHO cell line and purified the seperated protein.Methods:Human ?-NGF was transfected with lipofectamine reagent into CHO cell line.The biological activity was analyzed by objection with microscope and the method of MTT.The pure protein was proved by SDS-PAGE analysis.Results:The protein in the culture supernatant of the positive CHO cells transfected with ?-NGF gene could stimulate the growth of PC12 cell line and go into BBB.Conclusion:The target gene expressed successfully in the transfected CHO cell line and had good biological activity. [
