Construction of an Eukaryotic Expression Vector pcDNA3-Glutathione-S-Transferase A1 Gene From Human Liver cDNA Library and Partial Sequence Determination
- VernacularTitle:人谷胱甘肽硫转移酶A1真核表达系统的构建及序列分析
- Author:
Pingping CHEN
;
Yiming WU
;
Chaowu ZHANG
- Publication Type:Journal Article
- Keywords:
Glutathione-S-transferase A1;
RT-PCR;
Gene cloning expression;
Sequence determination
- From:
Journal of Environment and Health
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct an eukaryotic expression vector carrying human glutathione-S-transferase(GST)A1gene and to provide study materials for toxicology and pharmacogenetic.Methods The GST A1cDNA was amplified and separated from human liver total RNAs by RT-PCR approach and recombined with eukaryotic expression vector pcDNA3.The recombined plasmid pcDNA3-hGSTA1was verified using PCR,restriction analysis and sequencing determination.Results Human GST A1gene was recombined correctly with pcDNA3,compared with Genbank,in code152T→C,amino acid Met→Thr.Conclusion The eukaryotic expression vector pcDNA3-hGSTA1was constructed for research on toxicology and pharmacogenetics.
