The effect of NF-?B decoy on the chemokine expression of human bladder cancer cell line
- VernacularTitle:核因子?B诱骗剂对膀胱癌中趋化因子基因表达的影响
- Author:
Donghua XIE
;
Xiaoda TANG
;
Shujie XIA
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
NF- ?B;
Oligonucleotides;
Chemokine
- From:
Chinese Journal of Urology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of nuclear factor ?B(NF-?B)decoy on the chemokine expression in bladder cancer cell line. Methods Human bladder cancer cell line EJ,NF-?B decoy ODN were used as a NF-?B inhibitor(scrambled NF-?B decoy was used as control).NF-?B DNA binding activity was detected by electrophoretic mobility shift assay (EMSA);and p65 subunit of NF-?B was detected by RT-PCR and Western blot.Chemokines including IL-8,MCP-1,RANTES were detected by RT-PCR. Results EMSA showed that NF-?B decoy inhibited NF-?B activation induced by TNF-?.RT-PCR or Western blot test suggested that p65,IL-8,MCP-1 and RANTES were upregulated by TNF-? and downregulated by NF-?B decoy.However,mutated decoy ODN had no effect on them. Conclusions Chemokines can be detected in bladder cancer.They are activated by TNF-? and inhibited by NF-?B decoy.
