Inhibiting effect of endothelin A receptor antisense oligodeoxynucleotides on humun prostatic stromal cells proliferation
- VernacularTitle:内皮素受体A反义寡核苷酸对人前列腺间质细胞增殖的抑制作用
- Author:
Hengcheng ZHU
;
Gongcheng LU
;
Zhijian WU
- Publication Type:Journal Article
- Keywords:
Prostate;
Stromal cells;
Endothelin;
Receptor;
Antisense oligonucleotides
- From:
Chinese Journal of Urology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of endothelin A receptor antisense oligodeoxynucleotides (ETAR-ASODN)on the growth of human prostatic stromal cells. Methods Primary cultured prostatic stromal cells were derived from patients with benign prostatic hyperplasia (BPH).The cells of passages 4～6 were routinely used for this study after identification.ETAR-ASODN at the concentrations of 5,10 and 15 ?mol/L were added into the culture cells with lipofectin, and cell proliferation was measured by MTT assay. The expression of ETA receptor was tested by 125 I-ET-1 radioligand banding assay. Results MTT assays showed a significant decrease in cell proliferation in stromal cells after 5,10 and 15?mol/L ETAR-ASODN were added with the A 540 values being 0.304?0.082,0.296?0.008 and 0.194?0.061,respectively.The proliferative activity was significantly decreased compared with control group ( P
