Effect of estradiol on expressions of transforming growth factor ? and smoothelin in human prostatic stromal cells
- VernacularTitle:雌二醇对人前列腺间质细胞转化生长因子?与平滑肌细胞特异性抗原表达的影响
- Author:
Shaobin ZHENG
;
Wei TANG
;
Peng ZHANG
- Publication Type:Journal Article
- Keywords:
Prostate;
Cell,cultured;
Estradiol;
Transforming growth factor beta
- From:
Chinese Journal of Urology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationships among estrogen,transforming growth factor ?(TGF?) and smooth muscle cells in human prostatic tissue by cell experiment. Methods Eleven strains of prostatic stromal cells were cultured successfully from BPH patients,and stimulated by estradiol(E 2).Cell proliferations were observed by MTT,and mRNA expressions of TGF? 1,TGF? 2 and smoothelin were observed by RT-PCR. Results E 2 could not influence TGF? 1 expressions in prostatic stromal cells,but could upregulate expressions of TGF? 2 and smoothelin;and expression values of TGF? 2 and smoothelin had a positive correlation. Conclusions E 2 can influence growth and differentiation of prostatic smooth muscle cells through induction of TGF? 2 expression.
