The culture and identification of urinary tract smooth muscle cells
- VernacularTitle:尿路平滑肌细胞的培养和鉴定
- Author:
Xin ZHOU
;
Hongquan GENG
;
Xiangru WU
- Publication Type:Journal Article
- Keywords:
Urinary tract;
Smooth muscle;
Cells,cultured
- From:
Chinese Journal of Urology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of isolating,culturing,proliferating urinary tract(nephropelvics,ureter and bladder)smooth muscle cells (SMC). Methods Urinary tract SMC were isolated from sample tissues (1 from human nephropelvics,1 from human ureter,3 from human bladders,5 from porcine bladders and 1 from porcine ureter) by collagenase digestion and were cultured in DMEM supplemented with fetal bovine serum.Morphology and expansion of the cells were observed.SMC specific protein (?-actin) was identified by immunohistochemical methods. Results The cells grew well and presented a typical morphological feature of SMC.They could be passaged 6 times without a remarkable decrease in rate of cell proliferation.Immunohistochemical staining showed that ?-actin was positive. Conclusions This urinary tract SMC cultural method was simple,reliable and fruitful.
