Expression of activating transcription factor 5(ATF5) in renal cell carcinoma
- VernacularTitle:激活转录因子5在肾癌细胞系中的表达及意义
- Author:
Xiongjun YE
;
Zhiwen ZHANG
;
Guiting LIN
- Publication Type:Journal Article
- Keywords:
Kidney neoplasms;
Transcription factors;
Genes
- From:
Chinese Journal of Urology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the differential expression of ATF5--a novel gene fished out by yeast two hybrid from renal carcinoma library.MethodsRT-PCR and Northern blot was used to examine the expression level of ATF5 in renal cell line A498,786-O,GRC-I,293 and HK-2.The area absorbance value of ATF5 RNA was quantitative by computerized-imaging system.ResultsCompared with the controls,the expression level in renal cancer cell line(A498,786-O,GRC-I) and embryonal cell line (293)is much higher than that in the normal cell line HK-2.ConclusionsIt is indicated that ATF5 may play a significant role in the renal carcinogenesis through cross-talk with Wnt signal pathway.
